Journal: Frontiers in Veterinary Science

Article Title: Evaluation of the immunogenicity of a Crimean-Congo hemorrhagic fever virus vaccine candidate in mice developed based on a baculovirus Zera nanoparticle delivery system

doi: 10.3389/fvets.2023.1126785

Figure Lengend Snippet: Analysis of immunogenicity in mice. Analysis of induced IgG responses in mice by indirect ELISA for the Gn recombinant protein (A) and the Np recombinant protein (B) . The y-axis represents the OD450 nm of serum samples collected at 0, 14, 28, 35 and 42 d after immunization (DAI) for each group, *** p < 0.001, with significant differences from PBS and rvAc-dual (Bonferroni test). (C) Results of lymphocyte proliferation experiments. The y-axis represents the stimulation index of splenic lymphocyte samples collected at 35 and 42 DAI. ns: no significant difference; *** p < 0.001, significant difference (Bonferroni test). Quantitative analysis of IL-4 (D) and TNF-α (E) levels in the serum of immunized mice. All analyses were performed in triplicate, and error bars show the standard deviation (SD).

Article Snippet: TNF-α and IL-4 cytokines were detected in mouse sera by a commercial ELISA kit (Mouse IFN-γ/IL-4 ELISA BASIC kit Boster), and all sera to be tested were made in three replicates.

Techniques: Immunopeptidomics, Indirect ELISA, Recombinant, Standard Deviation